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Infections & SerologyNo Fasting RequiredTurnaround: 4–8 hours (same day)

Stool Routine & Occult Blood Examination (FOBT / R/M)

Also known as: Fecal Occult Blood Test (FOBT), Stool R/M, Stool Microscopy, Fecal Immunochemical Test (FIT)

Parameters Measured in the Report

A breakdown of individual biomarkers and biological reference intervals typically found in your report:

BiomarkerStandard RangeUnitWhat It Means
Fecal Occult Blood (FOBT / FIT)
(Occult Blood)
Negative (Nil)
qualitative / µg Hb/g

Detects microscopic blood leaking from polyps, ulcers, or tumors in the digestive tract.

Pus Cells / WBCs (Microscopy)
(Fecal Leukocytes)
Nil / 0–2
/HPF

Indicates invasive bacterial enteritis (Shigella, Salmonella) or active inflammatory bowel disease.

Red Blood Cells (Microscopy)
(Fecal RBCs)
Nil
/HPF

Microscopic bleeding from mucosal ulceration or severe mucosal inflammation.

Ova, Cysts & Parasites (Microscopy)
(O&P Screen)
Absent / None Seen
microscopy

Checks for intestinal parasites including Giardia, Entamoeba histolytica, hookworm, or roundworm.

* Note: Reference ranges may vary slightly between testing instruments and reagent kits. Always refer to the reference interval printed on your specific pathology report.

Clinical Focus

Microscopic presence of hidden (occult) hemoglobin, intestinal pus cells, red blood cells, ova, cysts, and parasites.

Sample & Specimen

Fresh stool specimen in clean sterile container

Fasting Protocol

No fasting required. Avoid red meat, turnips, and NSAIDs for 48 hours prior if using guaiac FOBT.

Turnaround Time

4–8 hours (same day)

What Is This Test?

A Stool Routine and Occult Blood Examination (FOBT / FIT) evaluates the digestive tract by analyzing feces for microscopic elements invisible to the naked eye. In gastrointestinal pathology, small bleeding ulcers or polyps often bleed in tiny quantities that do not visibly discolor stool, yet cause progressive iron deficiency anemia. The **Fecal Occult Blood Test** detects microscopic globin or heme molecules in stool. Combined with routine microscopic evaluation for protozoan cysts (*Entamoeba histolytica*, *Giardia lamblia*), helminth eggs, and pus cells, it differentiates inflammatory and parasitic diarrhea from functional irritable bowel syndrome.

Why Did Your Doctor Order This Test?

Physicians routinely prescribe the Stool Routine & FOBT in the following clinical scenarios:

Colorectal cancer screening in adults aged 45 to 75 as a non-invasive annual preliminary check
Investigating unexplained iron-deficiency anemia or chronic fatigue when upper/lower endoscopy is being evaluated
Chronic diarrhea, abdominal cramping, mucus in stool, or suspected intestinal amoebiasis or giardiasis
Differentiating acute infectious/inflammatory colitis from non-inflammatory functional bowel disorders

How to Prepare Before Your Test

Fasting Requirement: No fasting required. Avoid red meat, turnips, and NSAIDs for 48 hours prior if using guaiac FOBT.
  • Collect fresh stool in the clean, dry container provided. Do not contaminate with toilet bowl water or urine.
  • If taking a chemical guaiac FOBT: Avoid cooked red meat, broccoli, horseradish, Vitamin C supplements, and aspirin for 48 hours prior.
  • Do not collect sample during active hemorrhoidal bleeding or during menstrual flow.

Understanding Your Results

What higher or lower readings generally mean in clinical practice:

If Values Are Higher Than Normal

A positive Fecal Occult Blood test confirms gastrointestinal mucosal bleeding, requiring prompt clinical evaluation with colonoscopy and/or gastroscopy to identify or rule out colon polyps, colorectal carcinoma, diverticular disease, or peptic ulcers.

If Values Are Lower Than Normal

A negative stool routine and occult blood test indicates the absence of detectable GI hemorrhage and intestinal parasites in that specimen.

Frequently Asked Questions

Does a positive stool occult blood test mean I have colon cancer?

No. Most positive occult blood tests are caused by benign conditions like bleeding hemorrhoids (piles), anal fissures, stomach ulcers, diverticulosis, or colon polyps. However, it is an essential red-flag signal that warrants a colonoscopy.

Related Laboratory Tests

Tests frequently prescribed together or used as follow-up investigations:

Medical & Diagnostic Disclaimer

This guide is published for educational and patient informational purposes only. It is not a substitute for professional medical evaluation, diagnosis, or clinical advice.

A laboratory result that falls slightly outside standard statistical reference intervals does not automatically indicate disease; conversely, a normal result does not guarantee the absence of medical conditions. Only your attending physician can interpret lab findings in the context of your medical history, physical exam, and symptoms.

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